The study aimed to establish Complete Blood Count reference intervals (RIs) for the population served by the South-West Regional Health Authority, and determine if there were differences between the developed RIs and the currently used manufacturer RIs adopted from the Coulter Corporation.
A descriptive cross-sectional study was conducted at the Blood Bank Department of San Fernando General Hospital, Trinidad. Using convenience sampling, a total of 366 blood donors (198 males and 168 females) who passed the donor and health screening tests and contributed a unit of blood to the Blood Bank, agreed to have their residual blood used in the study.
Sampling collection and handling followed standard operating procedures. SPSS software was used for nonparametric statistical analysis such as the Wilcoxon Rank Sum Test and Pearson's Correlation. RIs were established for all participants after double-checking outliers with grossly abnormal test results. RIs for twenty parameters were developed which reflected red blood cells, white blood cells (WBC), and platelet values.
Differences were seen between the developed RIs and the manufacturer's RIs in all twenty parameters. The upper limits for WBC, neutrophil, and eosinophil were greater for males and females compared to the manufacturer's RIs. Haemoglobin lower limits for males and females (12.70 g/dL and 11.02 g/dL) respectively, were lesser than the manufacturer's RIs (13.60 g/dL and 11.70 g/dL). The observed differences may have been due to genetic adaptation, poor nutritional status, and increased exposure to allergens and parasitic infections. Population-specific RIs should be developed to improve patient treatment and management. Further investigations are required to determine if there are population differences within other blood test profiles.